For ab178846 Abcam recommends blocking in 3% milk for cleanest results in WB. Blocking with BSA gives slightly higher background.
Iba1 is a relatively minor protein of brain and is much more abundant in spleen (PMID: 8912632, PMID: 29232670). We suggest loading higher amount of brain lysate or using lower dilution of antibody for detecting signal in brain related lysates
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
WB: HL-60, THP-1, U937, RAW 264.7 and NR8383 whole cell lysates; Human, mouse and rat spleen lysates; Mouse testis and liver lysates; Rat and mouse hippocampus and brain tissue lysates. IHC-P: Human Cerebral cortex, human hippocampus; Rat and mouse normal brain tissues. Flow Cyt (intra): U937 cells, Raw264.7. ICC/IF: Rat hippocampal mixed glia.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope)；缓冲液配方: PBS and Azide；偶联物: Unconjugated
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